Preklapanje visoko pouzdanih jednomolekularnih očitanja

master's thesis

• Title (english): Overlapping Single Molecule High-Fidelity Sequencing Data
• Author: Dario Babojelić
• Mentor: Mile Šikić (mentor)
• Committee member: Mile Šikić (predsjednik povjerenstva)
• Committee member: Ana Sović Kržić (član povjerenstva)
• Committee member: Krešimir Križanović (član povjerenstva)
• Granter: University of Zagreb; Faculty of Electrical Engineering and Computing; Zagreb
• Defense date and country: 2020-07-13, Croatia
• Scientific / art field, discipline and subdiscipline: TECHNICAL SCIENCES; Computing

Abstract

Pacific Biosciences je nedavno predstavio novu tehnologiju za sekvenciranje dugačkih očitanja. Za razliku od dotadašnjih tehnologija za dugačka očitanja, nova jednomolekularna visoko pouzdana tehnologija stvara očitanja visoke točnosti koja se zato nazivaju \textit{HiFi (High-Fidelity)} očitanja. Trenutne metode za analizu DNA očitanja nisu prilagođene za rad nad visoko pouzdanim dugačkim očitanjima.
Tema ovog rada je pronalazak novih i nadogradnja starih metoda za pronalazak preklapanja i... More mapiranja koje će efikasnije koristiti nova visoko pouzdana očitanja. Less

Abstract (english)

Recently, Pacific Biosciences improved their sequencing chemistry and sequencer performance, which resulted in a novel protocol enabling high-fidelity (HiFi) fragments.
These single molecule real-time HiFi reads offer high accuracy while maintaining the length of longer reads.
Current state of the art software for analyzing DNA reads were not developed to utilize that.
The main subject of this thesis is exploiting the length and accuracy of HiFi reads. New methods, which adapt less... More sensitive approaches, for finding overlaps and mapping of DNA HiFi reads are explored and tested. Less

• Keywords: bioinformatika; DNA očitanja; preklapanja; mapiranje; PacBio; HiFi
• Keywords (english): bioinformatics; DNA reads; overlap; map; PacBio; HiFi; minimizer
• Language: english
• URN:NBN: urn:nbn:hr:168:619320
• Study programme: Title: Computing; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra inženjer/inženjerka računarstva (magistar/magistra inženjer/inženjerka računarstva)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Closed access
• Created on: 2023-01-13 11:42:05